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Product Name
TAF1 Polyclonal Antibody
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Description
Polyclonal antibody to TAF1
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Tested applications
WB
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Species reactivity
Human, Mouse
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Alternative names
TAF1 antibody; BA2R antibody; CCG1 antibody; CCGS antibody; DYT3 antibody; DYT3/TAF1 antibody; KAT4 antibody; MRXS33 antibody; N-TAF1 antibody; NSCL2 antibody; OF antibody; P250 antibody; TAF250 antibody; TAF2A antibody; TAFII-250 antibody; TAFII250 antibody; XDP antibody; TATA-box binding protein associated factor 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1630-1893 of human TAF1 (NP_004597.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - TAF1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using TAF1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
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Background
Largest component and core scaffold of the TFIID basal transcription factor complex. Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA-binding activity. Essential for progression of the G1 phase of the cell cycle. Exhibits histone acetyltransferase activity towards histones H3 and H4.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"