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Product Name
SYT17 antibody
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Description
SYT17 Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in human kidney tissue, A375 cells, human brain tissue, human testis tissue, mouse brain tissue, mouse lung tissue, PC-3 cells. Positive FC detected in HepG2 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human brain tissue, human lung cancer tissue. Observed molecular weight by Western-blot: 75-80kd
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Tested applications
ELISA, WB, IHC, IF, IP, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Protein B/K antibody; Synaptotagmin 17 antibody; synaptotagmin XVII antibody; SYT17 antibody; SytXVII antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SYT17 recombinant protein (Accession Number: NM_016524). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:115804(SYT17 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human brain using Catalog No:115804(SYT17 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using SYT17 antibody Catalog No:115804 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-SYT17 (IP:Catalog No:115804, 4ug; Detection:Catalog No:115804 1:800) with mouse brain tissue lysate 6000ug.
1X10^6 HepG2 cells were stained with 0.2ug SYT17 antibody (Catalog No:115804, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
The function of SYT17 has not been widely studied, and is yet to be fully elucidated. This antibody works well in WB, IHC and IF applications.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"