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Product Name
Syntaxin 17 antibody
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Description
Syntaxin 17 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human liver tissue. Positive IP detected in HepG2 cells. Positive WB detected in L02 cells, HepG2 cells, human testis tissue, mouse testis tissue. Observed molecular weight by Western-blot: 33 kDa
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Tested applications
ELISA, IHC, WB, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ20651 antibody; STX17 antibody; syntaxin 17 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Syntaxin 17 recombinant protein (Accession Number: XM_011518821). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
Immunohistochemical of paraffin-embedded human liver using Catalog No:115794(STX17 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:115794(STX17 antibody) at dilution of 1:50 (under 40x lens)
L02 cells were subjected to SDS PAGE followed by western blot with Catalog No:115794(STX17 Antibody) at dilution of 1:1000
IP Result of anti-STX17 (IP:Catalog No:115794, 4ug; Detection:Catalog No:115794 1:1000) with HepG2 cells lysate 3600ug.
Immunofluorescent analysis of HepG2 cells using Catalog No:115794(STX17 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. Syntaxin 17 (STX17) is a SNARE of the autophagosome involved in autophagy through the direct control of autophagosome membrane fusion with the lysosome membrane. STX17 may also play a role in the early secretory pathway where it may maintain the architecture of the endoplasmic reticulum-Golgi intermediate compartment/ERGIC and Golgi and/or regulate transport between the endoplasmic reticulum, the ERGIC and the Golgi.
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References
- Yang H, Peng YF, Ni HM. Basal Autophagy and Feedback Activation of Akt Are Associated with Resistance to Metformin-Induced Inhibition of Hepatic Tumor Cell Growth. PloS one. 10(6):e0130953. 2015.
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