Synstatin peptide
Not For Human Use, Lab Use Only.
Cat.#: 319052
Special Price 237.9 USD
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Product Name
Synstatin peptide
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Documents
Batch to batch variation of the purity
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Sequence Shortening
H-LPAGEKPEEGEPVLHVEAEPGFTARDKE-OH
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Sequence
H-Leu-Pro-Ala-Gly-Glu-Lys-Pro-Glu-Glu-Gly-Glu-Pro-Val-Leu-His-Val-Glu-Ala-Glu-Pro-Gly-Phe-Thr-Ala-Arg-Asp-Lys-Glu-OH
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Length (aa)
28
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Peptide Purity (HPLC)
95.33%
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Molecular Formula
C133H207N35O46
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Molecular Weight
3032.26
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Source
Synthetic
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Form
Powder
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Description
Synstatin is a peptide derived from amino acids 92-119 of the mouse syndecan sequence and is the active site for the αvβ3 and αvβ5 integrin activation mechanism. Syndecan is a critical regulator of these two integrins during angiogenesis and tumourigenesis, and this activation process can be blocked by the synstatin peptide. Synstatin displays an IC50 of 100-300 nM when used in vitro to inhibit αvβ3 dependent adhesion and cell migration on vitronectin or in aortic ring angiogenesis assays. Synstatin is a potent inhibitor of angiogenesis in vivo and blocks αvβ3 mediated tumour cell adhesion and migration and tumour formation in mouse models.
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Storage Guidelines
Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides
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References
- Beauvais et al (2009) Syndecan-1 regulates alphavbeta3 and alphavbeta5 integrin activation during angiogenesis and is blocked by synstatin, a novel peptide inhibitor. J Exp Med. 206(3) 691 PMID: 19255147
- Rapraeger (2013) Synstatin: a selective inhibitor of the syndecan-1-coupled IGF1R-αvβ3 integrin complex in tumorigenesis and angiogenesis. FEBS J. 280(10) 2207 PMID: 23375101
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About TFA salt
Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.
TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.
TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.
In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.
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Molar Concentration Calculator
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Dilution Calculator
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Percent Concentration Calculator
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
Related Products / Services
• Peptide Services: NovoPro's peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression.
• Standard Peptide Synthesis: NovoPro offers quality peptides at the most competitive prices in the industry, starting at $3.20 per amino acid. NovoPro provides PepBox – Automatic Quote Tool for online price calculation.
• Peptide Modifications: NovoPro offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"