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Product Name
Synaptotagmin-1 antibody
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Description
Synaptotagmin-1 Rabbit Polyclonal antibody. Positive IHC detected in human liver cancer tissue. Positive WB detected in human liver tissue, mouse testis tissue, Y79 cells. Positive IP detected in mouse testis tissue. Observed molecular weight by Western-blot: 65 kDa ,47 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
DKFZp781D2042 antibody; P65 antibody; SVP65 antibody; Synaptotagmin 1 antibody; synaptotagmin I antibody; SYT antibody; SYT1 antibody; SytI antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Synaptotagmin-1 recombinant protein (Accession Number: XM_047429483). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
human liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:115771(SYT1 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:115771(SYT1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:115771(SYT1 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-SYT1 (IP:Catalog No:115771, 4ug; Detection:Catalog No:115771 1:500) with mouse testis tissue lysate 4000ug.
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Background
The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). SYT1 (synaptotagmin I) was firstly identified as a 65-kDa protein with a wide distribution in neuronal and neurosecretory tissue (PMID: 7298720). Calcium binding to SYT1 participates in triggering neurotransmitter release at the synapse (PMID: 11242035). In addition to regulating exocytosis, SYT1 has also been implicated in endocytosis and neurite outgrowth (PMID: 15492212; 11078930; 10336114). This antibody recognizes endogenous SYT1, which has an experimentally determined molecular mass of 65-68 kDa. An additional band of 47 kDa could also be detected, which is corresponding to the calculated molecular weight of SYT1.
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References
- Zeng HL, Rao X, Zhang LK. Quantitative proteomics reveals olfactory input-dependent alterations in the mouse olfactory bulb proteome. Journal of proteomics. 109:125-42. 2014.
- Tian H, Wang L, Cai R, Zheng L, Guo L. Identification of protein network alterations upon retinal ischemia-reperfusion injury by quantitative proteomics using a Rattus norvegicus model. PloS one. 9(12):e116453. 2014.
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