-
Product Name
Synaptopodin antibody
- Documents
-
Description
Synaptopodin Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue, rat brain tissue. Positive IF detected in Hela cells, HepG2 cells. Positive IHC detected in mouse brain tissue. Observed molecular weight by Western-blot: 99-115 kDa
-
Tested applications
ELISA, WB, IF, IHC
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
KIAA1029 antibody; synaptopodin antibody; SYNPO antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of Synaptopodin recombinant protein (Accession Number: XM_024454351). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
-
Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:115769(SYNPO antibody) at dilution of 1:1000
Immunofluorescent analysis of HepG2 cells, using SYNPO antibody Catalog No:115769 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Immunohistochemistry of paraffin-embedded mouse brain tissue slide using Catalog No:115769(SYNPO Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemistry of paraffin-embedded mouse brain tissue slide using Catalog No:115769(SYNPO Antibody) at dilution of 1:200 (under 40x lens).
-
Background
Synaptopodin is a actin-associated protein expressed in mature dendritic spines and renal podocytes. Several isoforms of synaptopodin have been reported: the 100 kDa neural form and 110 kDa renal form. 44-45 kDa may represent the degradation product of synaptopodin. As a podocyte specific protein, synaptopodin expression level was decreased in kidney but increased in the urinary podocytes from patients with preeclampsia, indicating that synaptopodin is a useful marker for preeclampsia. (15841212, 10555072)
-
References
- Zhang QJ, He J, Ni W. Noninvasive urine-derived cell lines derived from neurological genetic patients. Neuroreport. 24(4):161-6. 2013.
- Wang Z, Liu J, Sun W. Effects of asiaticoside on levels of podocyte cytoskeletal proteins and renal slit diaphragm proteins in adriamycin-induced rat nephropathy. Life sciences. 93(8):352-8. 2013.
- Gao P, Meng XF, Su H. Thioredoxin-interacting protein mediates NALP3 inflammasome activation in podocytes during diabetic nephropathy. Biochimica et biophysica acta. 1843(11):2448-60. 2014.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"