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Product Name
Synaptojanin 2 antibody
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Description
Synaptojanin 2 Rabbit Polyclonal antibody. Positive WB detected in SH-SY5Y cells, PC-3 cells. Positive IF detected in SH-SY5Y cells. Positive IHC detected in human brain tissue. Observed molecular weight by Western-blot: 160 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
INPP5H antibody; KIAA0348 antibody; Synaptojanin 2 antibody; SYNJ2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Synaptojanin 2 recombinant protein (Accession Number: BC043277). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with Catalog No:115767(SYNJ2 antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human brain tissue slide using Catalog No:115767(SYNJ2 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human brain tissue slide using Catalog No:115767(SYNJ2 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed SH-SY5Y cells using Catalog No:115767(SYNJ2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
SYNJ2(Synaptojanin-2) is also named as KIAA0348 and belongs to the synaptojanin family. SYNJ2 encodes an inositol polyphosphate phosphatase that functions in recycling neurotransmitter vesicles and is implicated in spermatogenesis(PMID: 24103750). This protein has 3 isoforms produced by alternative splicing.
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