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Product Name
SYAP1 antibody
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Description
SYAP1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells, A431 cells. Positive IHC detected in human cervical cancer tissue, human small intestine tissue. Positive WB detected in HEK-293 cells, A431 cells, HepG2 cells, L02 cells, SMMC-7721 cells. Positive IP detected in L02 cells. Observed molecular weight by Western-blot: 50-55 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human; other species not tested.
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Alternative names
DKFZp686K221 antibody; FLJ14495 antibody; FLJ44185 antibody; PRO3113 antibody; SYAP1 antibody; Synapse assOCiated protein 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SYAP1 recombinant protein (Accession Number: NM_032796). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:115756(SYAP1 antibody) at dilution of 1:1000
IP Result of anti-SYAP1 (IP:Catalog No:115756, 4ug; Detection:Catalog No:115756 1:500) with L02 cells lysate 1800ug.
Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:115756(SYAP1 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:115756(SYAP1 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:115756(SYAP1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
SYAP1(Synapse-associated protein 1) is a human homologue of the Drosophila SAP47 (synapse associated protein), which is recognized by a monoclonal antibody that selectively stain synaptic terminals.This protein is a 352 amino acid protein that is ubiquitously expressed in adult tissues. SYAP1 contains one BSD domain which is is an approximately 60 amino acid long protein domain named after the BTF2-like transcription factors, Synapse-associated proteins and DOS2-like proteins in which it is found.
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