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Product Name
SUPT20H Polyclonal Antibody
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Description
Polyclonal antibody to SUPT20H
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
SUPT20H antibody; C13 antibody; C13orf19 antibody; FAM48A antibody; FP757 antibody; P38IP antibody; SPT20 antibody; transcription factor SPT20 homolog antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 580-779 of human SUPT20H (NP_001014308.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - SUPT20H Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SUPT20H antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
Immunohistochemistry - SUPT20H Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human colon using SUPT20H antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - SUPT20H Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human kidney using SUPT20H antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - SUPT20H Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse liver using SUPT20H antibody at dilution of 1:100 (40x lens).
Immunofluorescence - SUPT20H Polyclonal Antibody
Immunofluorescence analysis of A549 cells using SUPT20H antibody . Blue: DAPI for nuclear staining.
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Background
Required for MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) activation during gastrulation. Required for down-regulation of E-cadherin during gastrulation by regulating E-cadherin protein level downstream from NCK-interacting kinase (NIK) and independently of the regulation of transcription by FGF signaling and Snail (By similarity). Required for starvation-induced ATG9A trafficking during autophagy.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"