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Product Name
SUGCT Polyclonal Antibody
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Description
Polyclonal antibody to SUGCT
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
SUGCT antibody; C7orf10 antibody; DERP13 antibody; GA3 antibody; ORF19 antibody; succinyl-CoA:glutarate-CoA transferase antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human SUGCT (NP_001180240.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:200 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - SUGCT Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SUGCT antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunofluorescence - SUGCT Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using SUGCT antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Catalyzes the succinyl-CoA-dependent conversion of glutarate to glutaryl-CoA. Can use different dicarboxylic acids as CoA acceptors, the preferred ones are glutarate, succinate, adipate, and 3-hydroxymethylglutarate.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"