SUCLA2 antibody

Cat.#: 115818

Size:

Special Price 239.0 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    SUCLA2 antibody
  • Documents
  • Description
    SUCLA2 Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue, human heart tissue, human kidney tissue. Positive WB detected in PC-3 cells, HEK-293 cells, HepG2 cells, L02 cells, mouse brain tissue, mouse liver tissue, SH-SY5Y cells. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 48-50 kDa
  • Tested applications
    ELISA, WB, IHC, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    A BETA antibody; SCS betaA antibody; SUCLA2 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of SUCLA2 recombinant protein (Accession Number: NM_003850). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

    IHC: 1:20-1:200

  • Validations

    PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:115818(SUCLA2 antibody) at dilution of 1:500

    PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:115818(SUCLA2 antibody) at dilution of 1:500

    Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:115818(SUCLA2 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:115818(SUCLA2 antibody) at dilution of 1:100 (under 10x lens)

    IP Result of anti-SUCLA2 (IP:Catalog No:115818, 4ug; Detection:Catalog No:115818 1:1000) with HEK-293 cells lysate 2800ug.

    IP Result of anti-SUCLA2 (IP:Catalog No:115818, 4ug; Detection:Catalog No:115818 1:1000) with HEK-293 cells lysate 2800ug.

  • Background
    SUCLA2(succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial) is also named as SCS-betaA and belongs to the succinate/malate CoA ligase beta subunit family. It can catalyzes the ATP-dependent ligation of succinate and CoA to form succinyl-CoA.Defects in SUCLA2 are the cause of mitochondrial DNA depletion syndrome type 5 (MTDPS5).
  • References
    • Chinopoulos C, Gerencser AA, Mandi M. Forward operation of adenine nucleotide translocase during F0F1-ATPase reversal: critical role of matrix substrate-level phosphorylation. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 24(7):2405-16. 2010.
    • Kiss G, Konrad C, Doczi J. The negative impact of α-ketoglutarate dehydrogenase complex deficiency on matrix substrate-level phosphorylation. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 27(6):2392-406. 2013.
    • Dobolyi A, Ostergaard E, Bagó AG. Exclusive neuronal expression of SUCLA2 in the human brain. Brain structure & function. 220(1):135-51. 2015.
    • Dobolyi A, Bagó AG, Gál A. Localization of SUCLA2 and SUCLG2 subunits of succinyl CoA ligase within the cerebral cortex suggests the absence of matrix substrate-level phosphorylation in glial cells of the human brain. Journal of bioenergetics and biomembranes. 47(1-2):33-41. 2015.
    • Huang S, Cui Y, Guo X. 2,2',4,4'-Tetrabromodiphenyl ether disrupts spermatogenesis, impairs mitochondrial function and induces apoptosis of early leptotene spermatocytes in rats. Reproductive toxicology (Elmsford, N.Y.). 51:114-24. 2015.
    • Völgyi K, Gulyássy P, Háden K. Synaptic mitochondria: a brain mitochondria cluster with a specific proteome. Journal of proteomics. 120:142-57. 2015.
    • Carrozzo R, Verrigni D, Rasmussen M. Succinate-CoA ligase deficiency due to mutations in SUCLA2 and SUCLG1: phenotype and genotype correlations in 71 patients. Journal of inherited metabolic disease. 39(2):243-52. 2016.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"