Substance P peptide

Substance P peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

RPKPQQFFGLM

Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met

11

96.13%

C₆₃H₉₈N₁₈O₁₃S

1347.63

71977-09-8

Synthetic

Powder

Substance P (SP) is a key neuropeptide that mediates diverse physiological actions by binding to the seven-transmembrane domain neurokinin 1 receptor (NK1-R). Upon binding to NK1-R on the cell surface, SP triggers clathrin-dependent endocytosis, with the ligand-receptor complex internalized into early endosomes containing transferrin receptors (Tf-R). In acidified perinuclear vesicles, SP dissociates from NK1-R—while SP is slowly degraded intracellularly, the NK1-R recycles back to the plasma membrane, a process independent of new protein synthesis but requiring endosomal acidification. This endocytic and recycling pathway contributes to the desensitization and resensitization of cellular responses to SP, highlighting SP’s critical role in regulating receptor-mediated signaling and cellular adaptation.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Grady EF, Garland AM, Gamp PD, Lovett M, Payan DG, Bunnett NW. Delineation of the endocytic pathway of substance P and its seven-transmembrane domain NK1 receptor. Mol Biol Cell. 1995 May;6(5):509-24. doi: 10.1091/mbc.6.5.509. PMID: 7545030; PMCID: PMC301212.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.