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Product Name
SUB1 antibody
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Description
SUB1 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, A549 cells. Positive IHC detected in human pancreas cancer tissue. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 20 kDa,35 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
p14 antibody; PC4 antibody; Positive cofactor 4 antibody; RPO2TC1 antibody; SUB1 antibody; SUB1 homolog antibody; SUB1 homolog (S. cerevisiae) antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SUB1 recombinant protein (Accession Number: XM_047416661). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115816(SUB1 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:115816(SUB1 antibody) at dilution of 1:50 (under 25x lens)
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:115816(SUB1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
SUB1 gene encodes activated RNA polymerase II transcriptional coactivator p15, also termed as positive cofactor 4 (PC4) or p14. SUB1 is a sort of general coactivator that functions in assembling and stablization of transcription complex. It has DNA binding activity and is localized in the nucleus, mulitple modification sites of phosphorylation or acetylation may turn it appear slightly shifted molecular weight. Catalog#10948-2-AP is a rabbit polyclonal antibody raised against full-length human SUB1. This antibody recognize 14-16 kDa (P14 or P15), 20 kDa(phospho form) and 30 kDa (Dimer form).
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References
- Ho J, Kong JW, Choong LY. Novel breast cancer metastasis-associated proteins. Journal of proteome research. 8(2):583-94. 2009.
- Li L, Gong H, Yu H. Knockdown of nucleosome assembly protein 1-like 1 promotes dimethyl sulfoxide-induced differentiation of P19CL6 cells into cardiomyocytes. Journal of cellular biochemistry. 113(12):3788-96. 2012.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"