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Product Name
STXBP2 antibody
- Documents
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Description
STXBP2 Mouse Monoclonal antibody. Positive IHC detected in human lung cancer tissue. Positive IF detected in HepG2 cells. Positive WB detected in K-562 cells, fetal human brain tissue. Positive IP detected in K-562 cells. Observed molecular weight by Western-blot: 66 kDa
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Tested applications
ELISA, IF, IHC, IP, WB
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Species reactivity
Human; other species not tested.
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Alternative names
Hunc18b antibody; MUNC18 2 antibody; pp10122 antibody; STXBP2 antibody; STXBP2 protein antibody; syntaxin binding protein 2 antibody; UNC18 2 antibody; UNC18B antibody
- Immunogen
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of STXBP2 recombinant protein (Accession Number: NM_001127396). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:1000-1:10000
IHC: 1:50-1:500
IF: 1:50-1:500
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:107611(STXBP2 Antibody) at dilution of 1:4000
Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using Catalog No:107611(STXBP2 Antibody) at dilution of 1:400 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using Catalog No:107611(STXBP2 Antibody) at dilution of 1:400 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:107611(STXBP2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
IP Result of anti-STXBP2 (IP:Catalog No:107611, 5ug; Detection:Catalog No:107611 1:2000) with K-562 cells lysate 3200ug.
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Background
Syntaxin-binding protein 2 (STXBP2, also known as Unc18-2) belongs to the STXBP/unc-18/SEC1 family. STXBP2 is involved in intracellular trafficking, control of SNARE (soluble NSF attachment protein receptor) complex assembly, and the release of cytotoxic granules by natural killer cells. Mutations in the STXBP2 gene are associated with familial hemophagocytic lymphohistiocytosis.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"