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Product Name
STX5 Polyclonal Antibody
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Description
Polyclonal antibody to STX5
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Tested applications
WB
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Species reactivity
Rat
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Alternative names
STX5 antibody; SED5 antibody; STX5A antibody; syntaxin-5 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 55-355 of human STX5 (NP_003155.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - STX5 Polyclonal Antibody
Western blot analysis of extracts of rat brain, using STX5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
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Background
Mediates endoplasmic reticulum to Golgi transport. Together with p115/USO1 and GM130/GOLGA2, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"