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Product Name
STX1A Polyclonal Antibody
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Description
Polyclonal antibody to STX1A
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Rat
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Alternative names
STX1A antibody; HPC-1 antibody; P35-1 antibody; STX1 antibody; SYN1A antibody; syntaxin-1A antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-288 of human STX1A (NP_004594.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100 -
Validations
Western blot - STX1A Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using STX1A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.
Immunohistochemistry - STX1A Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver using STX1A antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - STX1A Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human gastric cancer using STX1A antibody at dilution of 1:100 (40x lens).
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Background
Plays a role in hormone and neurotransmitter exocytosis (By similarity). Potentially involved in docking of synaptic vesicles at presynaptic active zones. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm.
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