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Product Name
STRADA Polyclonal Antibody
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Description
Polyclonal antibody to STRADA
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Tested applications
WB
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Species reactivity
Human, Mouse
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Alternative names
STRADA antibody; LYK5 antibody; NY-BR-96 antibody; PMSE antibody; STRAD antibody; Stlk antibody; STE20-related kinase adaptor alpha antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 25-210 of human STRADA (NP_699166.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:1000 - 1:2000
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Validations
Western blot - STRADA Polyclonal Antibody
Western blot analysis of extracts of Mouse skeletal muscle, using STRADA antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
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Background
Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"