-
Product Name
STK4/MST1 antibody
- Documents
-
Description
STK4/MST1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human prostate cancer tissue, human prostate hyperplasia tissue. Positive WB detected in HeLa cells, rat liver tissue. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 56 kDa
-
Tested applications
ELISA, WB, IHC, IF, IP
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
DKFZp686A2068 antibody; KRS2 antibody; MST 1 antibody; MST1 antibody; serine/threonine kinase 4 antibody; STE20 like kinase MST1 antibody; STK4 antibody; YSK3 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of STK4/MST1 recombinant protein (Accession Number: NM_006282). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
-
Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115725(STK4 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:115725(STK4 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:115725(STK4 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:115725(STK4 Antibody) at dilution of 1:50 and and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-STK4 (IP:Catalog No:115725, 4ug; Detection:Catalog No:115725 1:1000) with HeLa cells lysate 1200ug.
-
Background
STK4 represents serine/threonine kinase 4
-
References
- Li L, Fang R, Liu B. Deacetylation of tumor-suppressor MST1 in Hippo pathway induces its degradation through HBXIP-elevated HDAC6 in promotion of breast cancer growth. Oncogene. 2015.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"