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Product Name
STIM1 antibody
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Description
STIM1 Mouse Monoclonal antibody. Positive IF detected in HEK-293 cells. Positive WB detected in HEK-293 cells, K-562 cells, RAW 264.7 cells. Observed molecular weight by Western-blot: 77 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Rat, Human, Mouse; other species not tested.
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Isotype
Mouse IgG2a
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Preparation
This antibody was obtained by immunization of STIM1 recombinant protein (Accession Number: NM_003156). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:107608(STIM1 Antibody) at dilution of 1:1000
Immunofluorescent analysis of HEK-293 cells using Catalog No:107608(STIM1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
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Background
STIM1, also named as GOK, is an ER Ca2+ sensor. Upon Ca2+ store depletion, STIM1 clusters at ER–plasma membrane junctions where it interacts with and gates Ca2+-permeable Orai1 ion channels. STIM1 is implicated in tumor growth suppression and stromal-haematopoietic cell interactions. Defacts in STIM1 gene can cause immune dysfunction with impaired T-cell activation. STIM1 can inhibit L-type voltage-gated Ca(2+) channels in neurons. Temperature is an important regulator of STIM1 function.The MW of STIM1 is about 80-90 kDa which may be due to the phosphorylation or glycosylation forms.
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