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Product Name
STAT5B antibody
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Description
STAT5B Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, human brain tissue, human liver tissue, Jurkat cells. Positive IP detected in HeLa cells. Positive IHC detected in human lung cancer tissue, human breast cancer tissue, human cervical cancer tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 90kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
STAT5 antibody; STAT5B antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of STAT5B recombinant protein (Accession Number: BC020868). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
WB result of anti-STAT5B (Catalog No:115694) in different cell lysates.
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115694(STAT5B antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:115694(STAT5B antibody) at dilution of 1:100 (under 10x lens)
IP Result of anti-STAT5B (IP:Catalog No:115694, 4ug; Detection:Catalog No:115694 1:500) with HeLa cells lysate 1200ug.
Immunofluorescent analysis of HepG2 cells using Catalog No:115694(STAT5B Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
STAT5B belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5B carries out a dual function: signal transduction and activation of transcription. STAT5B mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human STAT5B.
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References
- Zhang F, Yu X, He C. Effects of sexually dimorphic growth hormone secretory patterns on arachidonic acid metabolizing enzymes in rodent heart. Toxicology and applied pharmacology. 289(3):495-506. 2015.
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