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Product Name
STAT4 antibody
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Description
STAT4 Rabbit Polyclonal antibody. Positive IHC detected in human cervical cancer tissue, human testis tissue, mouse testis tissue, rat testis tissue. Positive IF detected in HeLa cells. Positive WB detected in HeLa cells, K-562 cells. Observed molecular weight by Western-blot: 86 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
SLEB11 antibody; STAT4 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: XM_006712719). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115690(STAT4 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:115690(STAT4 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:115690(STAT4 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HeLa cells using Catalog No:115690(STAT4 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
The JAK/STAT pathway is an extensive signaling pathway downstream of cytokine receptors. STATs are cytosolic proteins with a common structure consisting of an N-terminal oligomerization domain, which favors formation of STAT dimers, followed by a DNA-binding domain and a C-terminal SRC homology-2 (SH2) domain, which is involved in association between STATs and receptors[PMID:22383755]. Signal Transducer and Activator of Transcription 4 (STAT4) is a transcription factor that is activated by IL-12 signaling and promotes Th1-cell differentiation and IFN-γ production [PMID:21998209].
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