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Product Name
STAT3 antibody
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Description
STAT3 Rabbit Polyclonal antibody. Positive IHC detected in human cervical cancer tissue, human breast cancer tissue, human lung cancer tissue. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, K-562 cells. Observed molecular weight by Western-blot: 88kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
Acute phase response factor antibody; APRF antibody; FLJ20882 antibody; HIES antibody; STAT3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: XM_017024975). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115687(STAT3 antibody) at dilution of 1:400
IP Result of anti-STAT3 (IP:Catalog No:115687, 4ug; Detection:Catalog No:115687 1:500) with HeLa cells lysate 1500ug.
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:115687(STAT3 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:115687(STAT3 antibody) at dilution of 1:100 (under 40x lens)
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Background
Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human STAT3.
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References
- Gao X, Wang JY, Gao LM, Yin XF, Liu L. Identification and analysis of glycogen synthase kinase 3 beta1 interactome. Cell biology international. 37(8):768-79. 2013.
- Hu X, Yu J, Zhou X. A small GTPase‑like protein fragment of Mycoplasma promotes tumor cell migration and proliferation in vitro via interaction with Rac1 and Stat3. Molecular medicine reports. 9(1):173-9. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"