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Product Name
STAR Polyclonal Antibody
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Description
Polyclonal antibody to STAR
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
STAR antibody; STARD1 antibody; steroidogenic acute regulatory protein antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 64-285 of human STAR (NP_000340.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - STAR Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using STAR antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .
Immunofluorescence - STAR Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using STAR antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Plays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"