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Product Name
Stanniocalcin 2 antibody
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Description
Stanniocalcin 2 Mouse Monoclonal antibody. Positive WB detected in HEK-293 cells. Positive IHC detected in human breast cancer tissue, human kidney tissue, human stomach cancer tissue. Positive IF detected in Hela cells. Observed molecular weight by Western-blot: 33 kDa
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
stanniOCalcin 2 antibody; StanniOCalcin related protein antibody; STC 2 antibody; STC related protein antibody; STC2 antibody; STCRP antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of Stanni℃alcin 2 recombinant protein (Accession Number: NM_003714). Purification method: Caprylic acid/ammonium sulfate precipitation.
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Clonality
Monoclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:107605(STC2 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:107605(STC2 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:107605(STC2 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of Hela cells, using STC2 antibody Catalog No: at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
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Background
Stanniocalcin (STC) is a family of secreted glycoprotein hormones that originally discovered in the corpuscles of Stannius, an endocrine gland of fish. STC1 and STC2, two homologues of STC family, are reported to involve in calcium and phosphate homeostasis. It is expressed in a wide variety of tissues such as kidney, spleen, heart, and pancreas. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. STC2 overexpression could promote tumor cell proliferation, invasion and metastasis in prostate cancer, ovarian cancer or neuroblastoma. STC2 is also vital for cytoprotective properties when exposed to ER stress and hypoxia.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"