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Product Name
STAG2 antibody
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Description
STAG2 Rabbit Polyclonal antibody. Positive IF detected in 293. Positive IHC detected in human cervical cancer. Positive WB detected in Jurkat cells, HeLa cells, K-562 cells, MCF7 cells, NIH/3T3 cells. Observed molecular weight by Western-blot: 130-141kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
human, mouse, rat; other species not tested.
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Alternative names
STAG2 antibody; DKFZp686P168 antibody; FLJ25871 antibody; stromal antigen 2 antibody; SCC3 homolog 2 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of STAG2 recombinant protein (Accession Number: XM_017029234). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:115671(STAG2 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:115671(STAG2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:115671(STAG2 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of 293 cells, using STAG2 antibody Catalog No:115671 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
STAG2, also named as SA2, is a component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex may play a role in spindle pole assembly during mitosis. Mutations in STAG2 appear to be a first step in the transformation of a normal cell into a cancer cell.
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