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Product Name
ST8SIA4 Polyclonal Antibody
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Description
Polyclonal antibody to ST8SIA4
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Tested applications
WB
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Species reactivity
Human, Rat
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Alternative names
ST8SIA4 antibody; PST antibody; PST1 antibody; SIAT8D antibody; ST8SIA-IV antibody; CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferase antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-168 of human ST8SIA4 (NP_778222.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - ST8SIA4 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ST8SIA4 Antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 90s.
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Background
Catalyzes the polycondensation of alpha-2,8-linked sialic acid required for the synthesis of polysialic acid (PSA), which is present on the embryonic neural cell adhesion molecule (N-CAM), necessary for plasticity of neural cells.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"