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Product Name
ST6GALNAC4 Polyclonal Antibody
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Description
Polyclonal antibody to ST6GALNAC4
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Tested applications
WB
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Species reactivity
Human, Mouse
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Alternative names
ST6GALNAC4 antibody; IV antibody; SIAT3-C antibody; SIAT3C antibody; SIAT7-D antibody; SIAT7D antibody; ST6GALNACIV antibody; ST6GalNAc antibody; ST6 N-acetylgalactosaminide alpha-2,6-sialyltransferase 4 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 120-210 of human ST6GALNAC4 (NP_778204.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:1000 - 1:2000
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Validations
Western blot - ST6GALNAC4 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ST6GALNAC4 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
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Background
Involved in the biosynthesis of ganglioside GD1A from GM1B. Transfers CMP-NeuAc with an alpha-2,6-linkage to GalNAc residue on NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc of glycoproteins and glycolipids. Prefers glycoproteins to glycolipids (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"