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Product Name
ST3GAL1 Polyclonal Antibody
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Description
Polyclonal antibody to ST3GAL1
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Tested applications
WB
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Species reactivity
Mouse
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Alternative names
ST3GAL1 antibody; Gal-NAc6S antibody; SIAT4A antibody; SIATFL antibody; ST3GalA antibody; ST3GalA.1 antibody; ST3GalIA antibody; 1 antibody; ST3O antibody; ST3 beta-galactoside alpha-2,3-sialyltransferase 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 35-135 of human ST3GAL1 (NP_003024.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - ST3GAL1 Polyclonal Antibody
Western blot analysis of extracts of mouse liver, using ST3GAL1 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 40s.
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Background
Responsible for the synthesis of the sequence NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc- found on sugar chains O-linked to Thr or Ser and also as a terminal sequence on certain gangliosides. SIAT4A and SIAT4B sialylate the same acceptor substrates but exhibit different Km values.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"