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Product Name
ST13 Polyclonal Antibody
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Description
Polyclonal antibody to ST13
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Alternative names
ST13 antibody; AAG2 antibody; FAM10A1 antibody; FAM10A4 antibody; HIP antibody; HOP antibody; HSPABP antibody; HSPABP1 antibody; P48 antibody; PRO0786 antibody; SNC6 antibody; hsc70-interacting protein antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-369 of human ST13 (NP_003923.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - ST13 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ST13 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
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Background
One HIP oligomer binds the ATPase domains of at least two HSC70 molecules dependent on activation of the HSC70 ATPase by HSP40. Stabilizes the ADP state of HSC70 that has a high affinity for substrate protein. Through its own chaperone activity, it may contribute to the interaction of HSC70 with various target proteins (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"