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Product Name
SSR4 antibody
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Description
SSR4 Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human pancreas cancer tissue. Positive WB detected in HeLa cells, human placenta tissue. Observed molecular weight by Western-blot: 19kd
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Tested applications
ELISA, IHC, WB, IF
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Species reactivity
Human; other species not tested.
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Alternative names
SSR delta antibody; SSR4 antibody; TRAP delta antibody; TRAPD antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SSR4 recombinant protein (Accession Number: NM_006280). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115609(SSR4 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:115609(SSR4 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HeLa cells using Catalog No:115609(SSR4 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Signal sequence receptor subunit delta (SSR4, or TRAPD) gene encodes the translocon-associated proteins subunit delta, which is about 19-kDa and is located at endoplasmic reticulum (ER) membrane, the translocon-associated proteins complex functions to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. TRAP-delta can form heterotetramer with other TRAP homolog TRAP-alpha, TRAP-beta and TRAP-gamma.
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