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Product Name
SS18 Polyclonal Antibody
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Description
Polyclonal antibody to SS18
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Tested applications
WB, IHC, IF
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Species reactivity
Human
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Alternative names
SS18 antibody; SSXT antibody; SYT antibody; protein SSXT antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human SS18 (NP_001007560.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100 -
Validations
Western blot - SS18 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SS18 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunohistochemistry - SS18 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human colon carcinoma using SS18 antibody at dilution of 1:200 (40x lens).
Immunofluorescence - SS18 Polyclonal Antibody
Immunofluorescence analysis of MCF7 cells using SS18 antibody .
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Background
Appears to function synergistically with RBM14 as a transcriptional coactivator. Isoform 1 and isoform 2 function in nuclear receptor coactivation. Isoform 1 and isoform 2 function in general transcriptional coactivation. Component of SWI/SNF chromatin remodeling subcomplex GBAF that carries out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"