-
Product Name
SRSF4 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to SRSF4
-
Tested applications
WB, IF
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
SRSF4 antibody; SFRS4 antibody; SRP75 antibody; serine/arginine-rich splicing factor 4 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human SRSF4 (NP_005617.2).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - SRSF4 Polyclonal Antibody
Western blot analysis of extracts of SKOV3 cells, using SRSF4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - SRSF4 Polyclonal Antibody
Immunofluorescence analysis of MCF-7 cells using SRSF4 antibody . Blue: DAPI for nuclear staining.
-
Background
Plays a role in alternative splice site selection during pre-mRNA splicing. Represses the splicing of MAPT/Tau exon 10.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"