-
Product Name
SRPK1 antibody
- Documents
-
Description
SRPK1 Rabbit Polyclonal antibody. Positive FC detected in MCF-7 cells. Positive IF detected in SH-SY5Y cells. Positive IHC detected in human breast cancer tissue, human testis tissue. Positive IP detected in mouse testis tissue. Positive WB detected in Jurkat cells, L02 cells. Observed molecular weight by Western-blot: 95 kDa
-
Tested applications
ELISA, WB, FC, IF, IP, IHC
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
SFRS protein kinase 1 antibody; SFRSK1 antibody; SR protein specific kinase 1 antibody; SRPK1 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of SRPK1 recombinant protein (Accession Number: NM_003137). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
-
Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:115596(SRPK1 antibody) at dilution of 1:1000
IP Result of anti-SRPK1 (IP:Catalog No:115596, 3ug; Detection:Catalog No:115596 1:1000) with mouse testis tissue lysate 3000ug.
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:115596(SRPK1 Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:115596(SRPK1 Antibody) at dilution of 1:200 (under 40x lens).
Immunofluorescent analysis of SH-SY5Y cells using Catalog No:115596(SRPK1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 MCF-7 cells were stained with 0.2ug SRPK1 antibody (Catalog No:115596, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
-
Background
SRPK1 (serine-arginine protein kinase 1) is a protein kinase that specifically phosphorylates proteins containing serine-arginine-rich domains. Its substrates include a family of SR proteins that are key regulators of mRNA AS (alternative splicing). SRPK1 may be localized both in the cytoplasm and nucleus and translocates between these compartments under various conditions (e.g. cell cycle and stress). It is able to phosphorylate SR proteins both in cytoplasm and nucleus (the significance of which remains incompletely understood) and thus regulate the activity of these proteins by affecting their ability to bind other proteins and/or RNA as well as their intracellular localization.(PMID:22817743). This protein has 2 isoforms produced by alternative splicing.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"