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Product Name
SRP72 Polyclonal Antibody
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Description
Polyclonal antibody to SRP72
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Tested applications
WB, IHC, IF, FC
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Species reactivity
Human
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Alternative names
SRP72 antibody; BMFF antibody; BMFS1 antibody; HEL103 antibody; signal recognition particle 72 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human SRP72
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, pH7.3.
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Storage instructions
Store at 4℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:20 - 1:50
IF 1:20 - 1:50
FC 1:20 - 1:50 -
Validations
Western blot - SRP72 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SRP72 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
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Background
Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. Binds the 7S RNA only in presence of SRP68. This ribonucleoprotein complex might interact directly with the docking protein in the ER membrane and possibly participate in the elongation arrest function.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"