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Product Name
SREBF2 Polyclonal Antibody
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Description
Polyclonal antibody to SREBF2
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
SREBF2 antibody; SREBP-2 antibody; SREBP2 antibody; bHLHd2 antibody; sterol regulatory element-binding protein 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human SREBF2 (NP_004590.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:1000 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - SREBF2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SREBF2 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
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Background
Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the cholesterol and to a lesser degree the fatty acid synthesis pathway (By similarity). Binds the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') found in the flanking region of the LDRL and HMG-CoA synthase genes.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"