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Product Name
SREBF2 antibody
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Description
SREBF2 Rabbit Polyclonal antibody. Positive IHC detected in human kidney tissue, human brain tissue. Positive WB detected in Jurkat cells, HEK-293 cells, HeLa cells, HepG2 cells, human kidney tissue, mouse kidney tissue, rat kidney tissue, rat liver tissue. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 68 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
bHLHd2 antibody; SREBF2 antibody; SREBP 2 antibody; SREBP2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SREBF2 recombinant protein (Accession Number: NM_004599). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:115582(SREBF2 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human kidney using Catalog No:115582(SREBF2 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:115582(SREBF2 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-SREBF2 (IP:Catalog No:115582, 3ug; Detection:Catalog No:115582 1:500) with HeLa cells lysate 3000ug.
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Background
SREBF2, also named as BHLHD2, is a 1141 amino acid protein, which contains 1 bHLH domain and belongs to the SREBP family. SREBF2 localizes in the endoplasmic reticulum membrane and is ubiquitously expressed in adult and fetal tissues. SREBF2 as a transcriptional activator is required for lipid homeostasis. SREBF2 expression is dynamically regulated in response to nutritional and hormonal cues. The molecular weight of precursor isoform is 124 kDa and its mature isoform is 68 kDa (PMID:22657538).
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References
- Hu YW, Ma X, Huang JL. Dihydrocapsaicin Attenuates Plaque Formation through a PPARγ/LXRα Pathway in apoE(-/-) Mice Fed a High-Fat/High-Cholesterol Diet. PloS one. 8(6):e66876. 2013.
- Zhang S, Zheng L, Dong D. Effects of flavonoids from Rosa laevigata Michx fruit against high-fat diet-induced non-alcoholic fatty liver disease in rats. Food chemistry. 141(3):2108-16. 2013.
- Xu T, Zheng L, Xu L. Protective effects of dioscin against alcohol-induced liver injury. Archives of toxicology. 88(3):739-53. 2014.
- Fryer LG, Jones B, Duncan EJ. The endoplasmic reticulum coat protein II transport machinery coordinates cellular lipid secretion and cholesterol biosynthesis. The Journal of biological chemistry. 289(7):4244-61. 2014.
- Hu YW, Yang JY, Ma X. A lincRNA-DYNLRB2-2/GPR119/GLP-1R/ABCA1-dependent signal transduction pathway is essential for the regulation of cholesterol homeostasis. Journal of lipid research. 55(4):681-97. 2014.
- Yan H, Ma YL, Gui YZ. MG132, a proteasome inhibitor, enhances LDL uptake in HepG2 cells in vitro by regulating LDLR and PCSK9 expression. Acta pharmacologica Sinica. 35(8):994-1004. 2014.
- Hu YW, Zhao JY, Li SF. RP5-833A20.1/miR-382-5p/NFIA-dependent signal transduction pathway contributes to the regulation of cholesterol homeostasis and inflammatory reaction. Arteriosclerosis, thrombosis, and vascular biology. 35(1):87-101. 2015.
- Dong D, Qi Y, Xu L. Total saponins from Rosa laevigata Michx fruit attenuates hepatic steatosis induced by high-fat diet in rats. Food & function. 5(12):3065-75. 2014.
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