Cart 2

SRA1 antibody

Cat.#: 115662

Size:

Special Price 239.0 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    SRA1 antibody
  • Documents
  • Description
    SRA1 Rabbit Polyclonal antibody. Positive WB detected in MCF-7 cells, A2780 cells, HEK-293 cells. Positive IHC detected in human breast cancer tissue. Positive FC detected in MCF-7 cells. Observed molecular weight by Western-blot: 30 kDa, 33 kDa
  • Tested applications
    ELISA, WB, IHC, FC
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    pp7684 antibody; SRA antibody; SRA1 antibody; SRAP antibody; STRAA1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of SRA1 recombinant protein (Accession Number: BC067895). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:20-1:200

  • Validations

    MCF-7 cells were subjected to SDS PAGE followed by western blot with Catalog No:115662(SRA1 Antibody) at dilution of 1:1000

    MCF-7 cells were subjected to SDS PAGE followed by western blot with Catalog No:115662(SRA1 Antibody) at dilution of 1:1000

    Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:115662(SRA1 Antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:115662(SRA1 Antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:115662(SRA1 Antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:115662(SRA1 Antibody) at dilution of 1:50 (under 40x lens)

    1X10^6 MCF-7 cells were stained with 0.2ug SRA1 antibody (Catalog No:115662, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

    1X10^6 MCF-7 cells were stained with 0.2ug SRA1 antibody (Catalog No:115662, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

  • Background
    Steroid receptor RNA activator 1 (SRA1), also known as seroid receptor RNA activator protein (SRAP) or SRA, is involved in modulating the activity of multiple transcription factors including the estrogen receptor (ER) (PMID: 20398657). It may play a role in tumorigenesis (PMID: 16152589). The SRA1 gene encodes both SRA1 protein and a non-coding functional RNA that functions as part of a ribonucleoprotein complex activating steroid receptor induced transcription. This polyclonal antibody recognizes endogenous SRA1 isoforms which migrate as a doublet on SDS-PAGE gels (PMID: 12565891; 23907597).
  • References
    • Hu YW, Ma X, Huang JL. Dihydrocapsaicin Attenuates Plaque Formation through a PPARγ/LXRα Pathway in apoE(-/-) Mice Fed a High-Fat/High-Cholesterol Diet. PloS one. 8(6):e66876. 2013.
    • Hu YW, Yang JY, Ma X. A lincRNA-DYNLRB2-2/GPR119/GLP-1R/ABCA1-dependent signal transduction pathway is essential for the regulation of cholesterol homeostasis. Journal of lipid research. 55(4):681-97. 2014.
    • Hu YW, Zhao JY, Li SF. RP5-833A20.1/miR-382-5p/NFIA-dependent signal transduction pathway contributes to the regulation of cholesterol homeostasis and inflammatory reaction. Arteriosclerosis, thrombosis, and vascular biology. 35(1):87-101. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"