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Product Name
SPTLC1 Polyclonal Antibody
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Description
Polyclonal antibody to SPTLC1
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Tested applications
WB
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Species reactivity
Human, Mouse
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Alternative names
SPTLC1 antibody; HSAN1 antibody; HSN1 antibody; LBC1 antibody; LCB1 antibody; SPT1 antibody; SPTI antibody; serine palmitoyltransferase 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic Peptide of human SPTLC1
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, pH7.3.
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Storage instructions
Store at 4℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
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Validations
Western blot - SPTLC1 Polyclonal Antibody
Western blot analysis of extracts of HeLa cells, using SPTLC1 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
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Background
Serine palmitoyltransferase (SPT). The heterodimer formed with SPTLC2 or SPTLC3 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA. The SPTLC1-SPTLC2-SPTSSB complex shows a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme displays an ability to use a broader range of acyl-CoAs, without apparent preference. Required for adipocyte cell viability and metabolic homeostasis (By similarity).
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