-
Product Name
SPTLC1 antibody
- Documents
-
Description
SPTLC1 Rabbit Polyclonal antibody. Positive IP detected in HEK-293 cells. Positive WB detected in Jurkat cells, HEK-293 cells, HepG2 cells, human liver tissue. Positive IHC detected in human colon cancer tissue. Observed molecular weight by Western-blot: 55kd
-
Tested applications
ELISA, WB, IHC, IP
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
HSAN antibody; HSAN1 antibody; HSN1 antibody; LBC1 antibody; LCB 1 antibody; LCB1 antibody; Serine palmitoyltransferase 1 antibody; SPT 1 antibody; SPT1 antibody; SPTI antibody; SPTLC1 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of SPTLC1 recombinant protein (Accession Number: NM_178324). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
-
Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:115655(SPTLC1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:115655(SPTLC1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:115655(SPTLC1 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-SPTLC1 (IP:Catalog No:115655, 4ug; Detection:Catalog No:115655 1:500) with HEK-293 cells lysate 880ug.
-
Background
SPTLC1 represents serine palmitoyltransferase, long chain base subunit 1
-
References
- Geekiyanage H, Chan C. MicroRNA-137/181c regulates serine palmitoyltransferase and in turn amyloid β, novel targets in sporadic Alzheimer's disease. The Journal of neuroscience : the official journal of the Society for Neuroscience. 31(41):14820-30. 2011.
- Geekiyanage H, Upadhye A, Chan C. Inhibition of serine palmitoyltransferase reduces Aβ and tau hyperphosphorylation in a murine model: a safe therapeutic strategy for Alzheimer's disease. Neurobiology of aging. 34(8):2037-51. 2013.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"