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Product Name
SPTBN1-Specific antibody
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Description
SPTBN1-Specific Rabbit Polyclonal antibody. Positive IHC detected in human lung tissue, human kidney tissue. Positive IP detected in HEK-293 cells. Positive WB detected in mouse brain tissue, mouse kidney tissue, mouse lung tissue. Observed molecular weight by Western-blot: 275kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
Beta II spectrin antibody; beta-fodrin antibody; betaSpII antibody; ELF antibody; Fodrin beta chain antibody; Spectrin beta chain antibody; brain 1 antibody; SPTB2 antibody; SPTBN1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_003128). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:115653(SPTBN1-Specific antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human lung using Catalog No:115653(SPTBN1-Specific antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human lung using Catalog No:115653(SPTBN1-Specific antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-SPTBN1-Specific (IP:Catalog No:115653, 5ug; Detection:Catalog No:115653 1:600) with HEK-293 cells lysate 1000ug.
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Background
SPTBN1 is the non-erythrocytic form of β-spectrin or β-fodrin. Spectrins are members of the superfamily of F-actin cross linking proteins that are important as scaffolding proteins for protein sorting, cell adhesion, and migration. Reduced expression of SPTBN1 has been found to correlate with shorter survival of patients with hepatocellular cancer, pancreatic cancer and other malignancies of the gastrointestinal tract, suggesting tumor suppression ability of this protein.
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References
- Shen C, Yu Y, Li H. Global profiling of proteolytically modified proteins in human metastatic hepatocellular carcinoma cell lines reveals CAPN2 centered network. Proteomics. 12(12):1917-27. 2012.
- Niimori-Kita K, Ogino K, Mikami S. Identification of nuclear phosphoproteins as novel tobacco markers in mouse lung tissue following short-term exposure to tobacco smoke. FEBS open bio. 4:746-54. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"