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Product Name
SPTA1 Polyclonal Antibody
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Description
Polyclonal antibody to SPTA1
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
SPTA1 antibody; EL2 antibody; HPP antibody; HS3 antibody; SPH3 antibody; SPTA antibody; spectrin alpha, erythrocytic 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 940-1160 of human SPTA1 (NP_003117.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - SPTA1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SPTA1 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
Immunofluorescence - SPTA1 Polyclonal Antibody
Immunofluorescence analysis of rat bone marrow cells using SPTA1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - SPTA1 Polyclonal Antibody
Immunofluorescence analysis of mouse lung cells using SPTA1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"