-
Product Name
SPRR3 antibody
- Documents
-
Description
SPRR3 Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue, human breast cancer tissue. Positive IF detected in HeLa cells. Positive WB detected in HeLa cells, human liver tissue. Positive IP detected in COLO 320 cells. Observed molecular weight by Western-blot: 22-25 kDa
-
Tested applications
ELISA, WB, IHC, IP, IF
-
Species reactivity
Human; other species not tested.
-
Alternative names
22 kDa pancornulin antibody; Cornifin beta antibody; Esophagin antibody; small proline rich protein 3 antibody; SPRC antibody; SPRR3 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of SPRR3 recombinant protein (Accession Number: XM_047443702). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
-
Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115647(SPRR3 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:115647(SPRR3 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:115647(SPRR3 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-SPRR3 (IP:Catalog No:115647, 3ug; Detection:Catalog No:115647 1:500) with COLO 320 cells lysate 1720ug.
Immunofluorescent analysis of HeLa cells using Catalog No:115647(SPRR3 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
-
Background
SPRR3, also named as Esophagin, Cornifin beta and SPRC, belongs to the cornifin (SPRR) family. It is cross-linked envelope protein of keratinocytes. Increased expression of SPRR3 appears to be involved in colorectal tumorigenesis. SPRR3 is a marker for terminal squamous cell differentiation.a potential novel therapeutic target for less advanced stages of Breast Cancer.
-
References
- Cho DH, Jo YK, Roh SA. Upregulation of SPRR3 promotes colorectal tumorigenesis. Molecular medicine (Cambridge, Mass.). 16(7-8):271-7. 2010.
- Kim JC, Yu JH, Cho YK. Expression of SPRR3 is associated with tumor cell proliferation in less advanced stages of breast cancer. Breast cancer research and treatment. 133(3):909-16. 2012.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"