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Product Name
SPINT1/HAI-1 antibody
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Description
SPINT1/HAI-1 Rabbit Polyclonal antibody. Positive WB detected in human placenta tissue, Transfected HEK-293 cells. Positive IF detected in NIH/3T3 cells. Observed molecular weight by Western-blot: 58 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human; other species not tested.
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Alternative names
HAI antibody; HAI 1 antibody; HAI1 antibody; MANSC2 antibody; SPINT1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SPINT1/HAI-1 recombinant protein (Accession Number: NM_001032367). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IF: 1:50-1:500
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Validations
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:115574(SPINT1 antibody) at dilution of 1:100 (under 10x lens)
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:115574(SPINT1 Antibody) at dilution of 1:1000
human placenta tissue were subjected to SDS PAGE followed by western blot with Catalog No:115574(SPINT1 Antibody) at dilution of 1:300
Immunofluorescent analysis of (-20oc Ethanol) fixed NIH/3T3 cells using Catalog No:115574(SPINT1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
SPINT1 represents serine peptidase inhibitor, Kunitz type 1
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"