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SPERT antibody

Cat.#: 115564

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Product Information

  • Product Name
    SPERT antibody
  • Documents
  • Description
    SPERT Rabbit Polyclonal antibody. Positive IF detected in Hela cells. Positive WB detected in mouse testis tissue, mouse spermatophore tissue, PC-3 cells. Observed molecular weight by Western-blot: 48-52 kDa
  • Tested applications
    ELISA, WB, IF
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    CBY2 antibody; FLJ35810 antibody; NURIT antibody; Protein chibby homolog 2 antibody; spermatid assOCiated antibody; Spermatid assOCiated protein antibody; SPERT antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of SPERT recombinant protein (Accession Number: NM_001286342). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IF: 1:10-1:100

  • Validations

    mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:115564(SPERT antibody) at dilution of 1:1000

    mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:115564(SPERT antibody) at dilution of 1:1000

    Immunofluorescent analysis of Hela cells, using SPERT antibody Catalog No:115564 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    Immunofluorescent analysis of Hela cells, using SPERT antibody Catalog No:115564 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Background
    SPERT (spermatid associated protein), also known as CBY2 (chibby homolog 2) or NURIT, is a novel leucine-zipper protein belonging to the chibby family of proteins [PMID:12204287]. Uniquely Expressed in the spermatid flower-like structure, SPERT interacts with Nek1, a member of the NIMA-family kinase family that is associated centrosomal stability and ciliogenesis. It contains a leucine-zipper motif and two coiled-coil regions, and is transcribed through the elongation stage of the spermatids. SPERT is absent from mature spermatozoa and is thought to be involved in transporting proteins that are to be discarded via the residual bodies. [PMID:12204287]

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"