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Product Name
SPDYA Polyclonal Antibody
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Description
Polyclonal antibody to SPDYA
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Tested applications
WB, IF
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Species reactivity
Human
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Alternative names
SPDYA antibody; RINGO3 antibody; RINGOA antibody; SPDY1 antibody; SPY1 antibody; speedy protein A antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-313 of human SPDYA (NP_877433.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - SPDYA Polyclonal Antibody
Western blot analysis of extracts of A549 cells, using SPDYA antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 10s.
Immunofluorescence - SPDYA Polyclonal Antibody
Immunofluorescence analysis of MCF-7 cells using SPDYA antibody . Blue: DAPI for nuclear staining.
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Background
Regulates the G1/S phase transition of the cell cycle by binding and activating CDK1 and CDK2. Contributes to CDK2 activation without promoting CDK2 phosphorylation, by inducing a conformation change of the CDK2 T-loop that obstructs the substrate-binding cleft prior to kinase activation. Mediates cell survival during the DNA damage process through activation of CDK2.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"