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Product Name
SPATA7 Polyclonal Antibody
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Description
Polyclonal antibody to SPATA7
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
SPATA7 antibody; HEL-S-296 antibody; HSD-3.1 antibody; HSD3 antibody; LCA3 antibody; spermatogenesis associated 7 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 300-599 of human SPATA7 (NP_060888.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - SPATA7 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SPATA7 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
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Background
Involved in photoreceptor cells maintenance (By similarity). It is required for recruitment and proper localization of RPGRIP1 to the photoreceptor connecting cilium (CC), as well as protein trafficking across the CC to the outer segments (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"