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Product Name
SPATA22 antibody
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Description
SPATA22 Rabbit Polyclonal antibody. Positive IHC detected in human brain tissue. Positive WB detected in human testis tissue, mouse testis tissue. Positive IP detected in mouse testis tissue. Observed molecular weight by Western-blot: 41kd
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Tested applications
ELISA, WB, IP, IHC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
NYD SP20 antibody; SPATA22 antibody; spermatogenesis assOCiated 22 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SPATA22 recombinant protein (Accession Number: NM_001170698). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
human testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:115552(SPATA22 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human brain using Catalog No:115552(SPATA22 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:115552(SPATA22 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-SPATA22 (IP:Catalog No:115552, 3ug; Detection:Catalog No:115552 1:500) with mouse testis tissue lysate 4800ug.
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Background
Spata22 (NYD-SP20), a novel vertebrate-specific gene, is required for meiotic progress in mouse germ cells. It is essential for one or more key events of early meiotic prophase, as homologous chromosomes of mutant germ cells do not achieve normal synapsis or repair meiotic DNA double-strand breaks.
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References
- Luo M, Yang F, Leu NA. MEIOB exhibits single-stranded DNA-binding and exonuclease activities and is essential for meiotic recombination. Nature communications. 4:2788. 2013.
- La Salle S, Palmer K, O'Brien M, Schimenti JC, Eppig J, Handel MA. Spata22, a novel vertebrate-specific gene, is required for meiotic progress in mouse germ cells. Biology of reproduction. 86(2):45. 2012.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"