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Product Name
Human Osteonectin / SPARC (His Tag) recombinant protein
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Description
Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
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Protein name
SPARC
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Protein short names
OSTEONECTIN; CYSTEINE-RICH PROTEIN; BM-40; ON; SPARC; AA517111; RP23-465I4.1
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Uniprot ID
P09486
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Gene Name
SPARC; ON
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the human SPARC (NP_003109.1) precursor (Met 1-Ile 303) with a carboxy-terminal polyhistidine tag was expressed.
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Protein Species
Human
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Molecular weight
The secreted recombinant human SPARC consists of 297 amino acids with the predicted molecular mass of 34 kDa. As a result of glycosylation, rhSPARC migrates as an approximately 45 kDa band in SDS-PAGE under reducing conditions.
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Purity
> 98 % as determined by SDS-PAGE
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Validations
Human Osteonectin / SPARC Protein (His Tag) SDS-PAGE
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