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Product Name
SPANXE antibody
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Description
SPANXE Rabbit Polyclonal antibody. Positive IHC detected in human testis tissue. Positive IF detected in A375 cells. Positive WB detected in A375 cells, mouse skin tissue. Observed molecular weight by Western-blot: 15-20 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
SPANX A2 antibody; SPANX C antibody; SPANX D antibody; SPANX E antibody; SPANX family member E antibody; SPANX family antibody; member E antibody; SPANXA2 antibody; SPANXD antibody; SPANXE antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SPANXE recombinant protein (Accession Number: NM_022661). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
A375 cells were subjected to SDS PAGE followed by western blot with Catalog No:115542(SPANXE antibody) at dilution of 1:300
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:115542(SPANXE Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of A375 cells using Catalog No:115542(SPANXE Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
SPANXE (Sperm protein associated with the nucleus on the X chromosome E), also known as SPANX A2 or SPANX C, is a member of the SPANX family. The SPANX genes encode differentially expressed testis-specific proteins that localize to various subcellular compartments. This particular gene encodes a sperm protein that contains a consensus nuclear localization signal but, although a role in spermatogenesis is suggested, the specific function of this family member has not yet been determined.
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