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Product Name
SPAM1 Polyclonal Antibody
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Description
Polyclonal antibody to SPAM1
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
SPAM1 antibody; HEL-S-96n antibody; HYA1 antibody; HYAL1 antibody; HYAL3 antibody; HYAL5 antibody; PH-20 antibody; PH20 antibody; SPAG15 antibody; hyaluronidase PH-20 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 100-300 of human SPAM1 (NP_003108.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - SPAM1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SPAM1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence - SPAM1 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using SPAM1 antibody . Blue: DAPI for nuclear staining.
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Background
Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"